Biotin his-tag

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August undefined, 2024

WebProduct Details. Monoclonal mouse Anti·His Antibodies (5xHis antibodies and 4xHis antibodies), like the RGS·His Antibody, BSA-free, Tetra·His Antibody, BSA-free and Penta·His Antibody, BSA-free are used to detect recombinant protein carrying His tags. Each of the three variants detects a different His tag epitope. WebHis-tag is one of the most widely applied tags for recombinant protein expression and purification, and also used for binding assay to detect protein-protein interactions by a …

Cys-Streptavidin (His-tag) - Click Biosystems Inc.

WebAll lanes : Biotin Anti-6X His tag® antibody [HIS.H8] (ab173828) at 1/1000 dilution Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 0.5 µg Lane 2 : E. coli lysate … WebHHHHHH. Specificity. This protein-A purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. fix witgoed

Molecular Tag, biotin histag, FISH probe - cytomol.com

WebDescription. Recombinant human Ubiquitin (polyubiquitin-C), encompassing amino acids 2-76. This construct contains an N-terminal His-tag (6xHis) followed by an Avi-Tag™. The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified. . WebCys-Streptavidin is a recombinant streptavidin for site-specific conjugation. Like the native streptavidin, Cys-Streptavidin has four identical subunits and binds specifically to biotin with an extremely high affinity. However, the subunit of the Cys-Streptavidin protein has a single cysteine residue at N-terminus for direct conjugation. http://resources.bio-techne.com/images/research/Avi-tag-Application-Note-RSD20-8876.pdf cannon beach gun shop

Stable immobilisation of His-tagged proteins on BLI …

AlphaScreen Biotinylated-HIS PerkinElmer

Webafﬁ nity (C). In this experiment, recombinant human PD-L1/B7-H1 His-tag Avi-tag biotinylated protein was captured at a low coupling density to the active ﬂ ow cell via the Avi-tag biotin. Then, recombinant PD-1 His-tag protein at a concentration range between 3.2 nM and 13.2 uM was passed over both active and uncoupled reference ﬂ ow cells WebAntibodies that detect 6x His Tag can be used in several scientific applications, including Western Blot, Immunocytochemistry, ELISA, Immunoprecipitation and Immunohistochemistry. Our 6x His Tag monoclonal, polyclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Mouse, Rabbit, Goat, Sheep … fix wires to wallWebRecombinant Human MuSK Avi-tag His-tag Biotin Protein, CF. Cat # AVI10189. Human TfR2 Alexa Fluor® 647-conjugated Antibody. Cat # FAB31202R. Recombinant S. pyogenes CRISPR-associated Protein 9, CF. Cat # 9957-C9. Recombinant Human B7-2/CD86 Fc Chimera Avi-tag, CF. Cat # AVI7625. cannon beach haystack rock pictures

"WebJun 14, 2007 · The Strep-tag II is an eight-residue minimal peptide sequence (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys) that exhibits intrinsic affinity toward streptavidin and can be fused to recombinant proteins in ... " - Biotin his-tag

Biotin his-tag

WebAnti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western ... WebHis-tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe. Our His-tag antibodies also …

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WebBiotin-binding plates: Streptavidin or neutravidin: Binds small biotinylated peptides and other small molecules that are difficult to bind by passive adsorption: Fusion-tag binding plates: Glutathione (GST tag binding) or … WebDescription. Recombinant human Ubiquitin (polyubiquitin-C), encompassing amino acids 2-76. This construct contains an N-terminal His-tag (6xHis) followed by an Avi-Tag™. The …

Webafﬁ nity (C). In this experiment, recombinant human PD-L1/B7-H1 His-tag Avi-tag biotinylated protein was captured at a low coupling density to the active ﬂ ow cell via the … WebIn a functional flow cytometry test, (A) Recombinant SARS-CoV-2 Spike His-tag Biotin Protein (Catalog # BT10549; 500 ng/ml) binds to HEK293 human embryonic kidney cell line transfected with recombinant human …

WebKeywords: His-tagged protein, Ni-NTA, biolayer interferometry, avidin-biotin, norovirus, non-labelled detection 1. Introduction Histidine tags, e.g. insertion of a stretch of 5-10 histidine amino acids in either the N- or C-terminus of a recombinant protein, are typically used as affinity tags for purification purposes [1-3]. WebHis-tag is one of the most widely applied tags for recombinant protein expression and purification, and also used for binding assay to detect protein-protein interactions by a pull-down assay or in conjunction with anti-His antibody as IP/Co-IP assay. ... THE™ His Tag Antibody [Biotin], mAb, Mouse: $425.00 100 μg A01620. THE™ His Tag ...

http://resources.bio-techne.com/images/research/Avi-tag-Application-Note-RSD20-8876.pdf

WebMonoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH. Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol. The antibody was purified by affinity chromatography. The antibody solution should be stored undiluted between 2°C and 8°C. fix wire on anti-static wrist strapWebof His-tags using Ni2+-NTA (nitrilotriacetic acid) columns, and secondly, the pH-dependent binding of iminobiotin to strep-tavidin (basic pH, K d 1110 M; acidic pH, K d 10 3 M).13 In this method, varying numbers of biotin or iminobiotin conju-gated His-tags (Bio-His-Tag or Ibio-His-Tag, respectively) were rst introduced on to streptavidin (Fig. 1). fix wiring taskWebAnti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X His … cannon beach cottage flowersWebbiotechrabbit™ Tris-NTA Biotin for high-affinity His-tag binding. His-tags are one of the most commonly used tags for protein expression analysis. Conventional metal ion … fix wiring bose speakersWebCommonly modified sequences, or tags include 6xHIS-tag and FLAG ®-tag, or the addition of GST, MBP, and SUMO domains. The addition of biotin and biotinylated substances to … cannon beach haystack rock webcamWebRecombinant Protein A/G, His-tag. >96% by SDS-PAGE and RP-HPLC analyses. The recombinant Protein A/G is a genetically engineered protein containing 5 IgG-binding regions of protein A and 3 of protein G. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein … fix with goldWebBiotin-labeled anti-His-tag antibodies are used to indirectly label proteins tagged with 6XHis. This can be advantageous when working with partially purified protein, or in cases … cannon beach haystack rock oregon