Topflash reporter system
WebThe TCF/LEF Reporter kit is designed for monitoring the activity of Wnt / β-catenin signaling pathway in the cultured cells. The kit contains transfection-ready TCF/LEF luciferase … WebThis is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold …
Topflash reporter system
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WebTOPflash: Transfection grade T-cell factor (TCF) reporter plasmid containing two sets (with the second set in the reverse orientation) of three copies of the TCF binding site (wild … WebTOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF …
WebLuciferase reporter assays allow you to investigate promoter activity by measuring light output from luciferase enzyme that is expressed under the control of your promoter of interest. Luciferase genes vary in stability, light output and spectral characteristics, and reporter kits are available with different luciferases for both single- and dual-spectral … WebThe Luciferase Reporter 1000 Assay System (Cat.#. E4550) was designed to meet the needs of users who perform a large number of assays, particularly in 96-well plates. The system contains sufficient reagents to perform 1,000 luciferase assays (100µl per assay). For users working with transformed cells, a cell lysis buffer will be needed for ...
WebTOPFlash/FOPFlash assays. TOPFlash is a luciferase reporter that contains a minimal fos promoter coupled to Tcf-binding sites upstream of a modified firefly luciferase gene. … WebTOPFlash luciferase reporter which has binding sites for. TCF Reporter Plasmid Kit four of transfection grade T cell factor TCF reporter plasmids for over in TOPFlash and FOPFlash wntb-catenin activity assays. -CateninLEF1 transactivates the microRNA-371-373 cluster. Ecm from promega kit from the topflash reporter assay protocol with.
WebBT-549 cells were transiently transfected with TOP-FLASH firefly luciferase reporter vector and Renilla luciferase vector. After 24 hour-transfection, cells were treated with DMSO or 25 μM iCRT-3 for 48 hours. Luciferase activities were …
WebThe artificial TOP reporter system is a widely used tool in the investigation of Wnt/β-catenin signaling, including genomic and drug screens (42, 43). However, the relevance of this … lincolnshire house brumby wood laneWebThe Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Linear results are seen over at least eight orders of magnitude of … hotels with lazy rivers pensacola beachWebTOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF … lincolnshire house scunthorpeWebThe Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Linear results are seen over at least eight orders of magnitude of enzyme concentration, and less than 10 –20 moles of luciferase can be … hotels with lodges in grounds ukWeb16. máj 2024 · The cell line, which contains a luciferase reporter under control of 7 LEF/TCF binding sites, utilizes the Super TOPFlash system. The cells have been shown by the … lincolnshire house for saleWebThis is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold (activation by phosphorylation mutants of beta-catenin). The appropriate control plasmid is clone M51, Super8XFOPflash, which has mutant TCF/LEF binding sites. lincolnshire household waste sitesWeb21. feb 2012 · TOPflash is a TCF reporter plasmid containing two sets of three copies of wild-type TCF binding sites driven by the thymidine kinase minimal prompter and … hotels with living room near me